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A chronic user is someone who uses a substance regularly and over an extended period often daily or near-daily for months or longer

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The threshold intensity was manually set and kept constant, and the number of pixels was determined for 4G8-, ThS-, Iba1- and GFAP stained sections to quantify amyloid load, fibrillar burden, microglial activation and reactive astrogliosis in hippocampal (CA1, CA2, CA3 and dentate gyrus), subiculum and cortical areas (including motor, somatosensory, visual, frontal, parietal and retrosplenial cortex)

But in some countries, the amount can change because pack sizes are different from others